diff --git a/var/spack/repos/builtin/packages/breseq/package.py b/var/spack/repos/builtin/packages/breseq/package.py
index 6e406fba6aa..922ea73b8e0 100644
--- a/var/spack/repos/builtin/packages/breseq/package.py
+++ b/var/spack/repos/builtin/packages/breseq/package.py
@@ -14,6 +14,7 @@ class Breseq(AutotoolsPackage):
     homepage = "http://barricklab.org/breseq"
     url      = "https://github.com/barricklab/breseq/archive/v0.31.1.tar.gz"
 
+    version('0.33.1', sha256='e24a50e254ad026c519747313b9e42bbeb32bd766a6a06ed369bd5b9dc50e84d')
     version('0.31.1', 'a4e602d5481f8692833ba3d5a3cd0394')
 
     depends_on('autoconf', type='build')