diff --git a/var/spack/repos/builtin/packages/deconwolf/package.py b/var/spack/repos/builtin/packages/deconwolf/package.py
new file mode 100644
index 00000000000..b1c5050580c
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+++ b/var/spack/repos/builtin/packages/deconwolf/package.py
@@ -0,0 +1,33 @@
+# Copyright Spack Project Developers. See COPYRIGHT file for details.
+#
+# SPDX-License-Identifier: (Apache-2.0 OR MIT)
+
+
+from spack.package import *
+
+
+class Deconwolf(CMakePackage):
+    """deconwolf is a software for 3-D deconvolution of fluorescent wide-field images."""
+
+    homepage = "https://deconwolf.fht.org/"
+    url = "https://github.com/elgw/deconwolf/archive/refs/tags/v0.4.5.tar.gz"
+    git = "https://github.com/elgw/deconwolf.git"
+
+    maintainers("dacolombo")
+
+    license("GPL-3.0-only")
+
+    version("0.4.5", tag="v0.4.5", commit="ca062c49eedd6bf60fcd9804df467e64cfc3c113")
+
+    patch(
+        "https://github.com/elgw/deconwolf/commit/c5f28dd3d6ef532b6963127e551da1d7b4f33f83.patch?full_index=1",
+        sha256="d67096f7d2c7ddc764884e3cb09b0a6bbbca7eca5253512e73bc8d7eff89a1fc",
+        when="@0.4.5",
+    )
+
+    depends_on("cmake@3.22:")
+    depends_on("fftw@3")
+    depends_on("libtiff")
+    depends_on("gsl")
+    depends_on("libpng@1.6:")
+    depends_on("opencl")